Billions of people still suffer from hunger and food insecurity worldwide. Meanwhile, microbial contamination causes a third of global food waste annually, resulting in major economic losses and illnesses. Although antibiotics and pesticides help control contamination, their excessive use can promote bacterial resistance or leave toxic residues.
One alternative uses materials that generate highly reactive forms of oxygen when exposed to light, called photocatalysts, to prolong food freshness. These molecules kill bacteria by damaging their cell components in a process called photodynamic inactivation or PDI. However, current PDI systems rely on energy-intensive lasers and long exposure times because the bacteria’s protective cell membranes block reactive oxygen molecules. As a result, the systems don’t kill bacteria effectively and risk overheating food, affecting its freshness and nutritional value.
Scientists at Zhejiang University in China thought that it would be possible to achieve a PDI system using accessible, free sunlight with the right photocatalyst. Moreover, they also wanted to integrate this photocatalyst into food packaging to combat microbes during storage.
To make the photocatalyst, the researchers mixed water with a glue-like plant fiber that provides structural support, called pectin, and a chemical from plant seeds that supplies phosphorus, called phytic acid. Then they heated the mixture in a sealed reactor at 220ºC (428ºF) for 5 hours to form tiny structures composed of a carbon core surrounded by a phosphorus-rich shell called phosphorus-modified carbonized polymer dots or PCDs.
To incorporate the PCDs into film packaging, the researchers dissolved equal amounts of a plant fiber from konjac, called glucomannan, and another plant-derived molecule, called carboxymethylcellulose, in warm water to form a network of entangled molecular chains. Then, they added 1.5 milligrams of PCD per milliliter to this mixture, which linked the chains together like molecular fasteners. Finally, they added a moisture-binding chemical called glycerin to prevent brittleness. The researchers cast this mixture onto separate Petri dishes and dried them, forming thin, plastic-like PCD films.
To test the films, the researchers placed Escherichia coli bacteria between 2 sterilized film sheets and exposed the samples to either darkness or simulated sunlight for 20 minutes. They then transferred the microbes onto agar plates and incubated them for 15 hours, allowing surviving bacteria to form visible colonies. They compared the number of bacterial colonies with those of untreated samples to calculate the percentage of bacteria the films eliminated in each environment.
Tests under darkness showed that the films eliminated about 60% of the bacteria. The researchers attributed this result to the positive electrical charge of PCDs, which attracts the bacteria’s negatively charged cell surfaces. The electrical attraction stresses their cell membranes, interfering with essential life processes. This result suggested that the films use a separate charge-based antibacterial mechanism apart from PDI, which requires light to work.
Under simulated sunlight, the films eliminated nearly 100% of the bacteria. The researchers identified 2 principal mechanisms causing this. First, the PCDs acted as tiny heaters that generated localized heat, which stretched holes in the bacterial membranes, allowing contents to leak out of their cells through a process called photoporation. Second, the PCDs generated reactive oxygen molecules that could then penetrate the bacteria. Once inside the cells, these molecules irreversibly damage the bacteria’s internal components and genetic material through PDI.
The researchers also investigated whether PCD films could serve as food packaging by using them to wrap fresh strawberries. Compared with conventional nonbiodegradable polyethylene packaging where visible rotting occurred, the PCD films kept the strawberries fresh for more than 4 days by suppressing bacterial growth and regulating moisture levels. When the team tested whether the films were toxic, they found that the films are safe for humans and compatible for food applications. They also buried the films in soil for 24 days and found that they degraded by 78%, demonstrating biodegradability suitable for packaging fresh foods.
The team concluded that PCD films could be a biodegradable food packaging that combines plant-based materials with multiple antibacterial strategies. The films retained antibacterial activity in darkness and became more effective under sunlight through the combined effects of photoporation and PDI. These findings demonstrated that by combining multiple mechanisms into a single material, researchers can create multifunctional food packaging that remains effective across different storage conditions.
